D5319
Deoxyribonuclease I bovine
recombinant, expressed in Pichia pastoris, buffered aqueous glycerol solution, ≥5,000 units/mg protein
Sinónimos:
DNAse I, Deoxyribonucleate 5′-oligonucleotido-hydrolase
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About This Item
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
Productos recomendados
origen biológico
bovine
Nivel de calidad
recombinante
expressed in Pichia pastoris
Análisis
≥95%
formulario
buffered aqueous glycerol solution
actividad específica
≥5,000 units/mg protein
mol peso
~39 kDa
técnicas
DNA extraction: suitable
idoneidad
suitable for molecular biology
aplicaciones
diagnostic assay manufacturing
actividad extraña
RNAse and protease, free
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
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Aplicación
DNAse I from Sigma was used to treat nuclear lysate to obtain single nucleosomes in a study. The enzyme has also been for the preparation and harvest of mice mammary glands.
Deoxyribonuclease I bovine has been used in a study to compare the initial actions of spleen deoxyribonuclease and pancreatic deoxyribonuclease. Deoxyribonuclease I bovine has also been used in a study to investigate deoxythymidine 3′, 5′-di-p-nitrophenyl phosphate as a synthetic substrate for bovine pancreatic deoxyribonuclease.
Used for the removal of DNA from protein samples.
Acciones bioquímicas o fisiológicas
DNase I is an endonuclease that acts on phosphodiester bonds (adjacent to pyrimidines) to produce polynucleotides with terminal 5′-phosphates. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.
Digests single- and double-stranded DNA to a mixture of mono- and oligonucleotides carrying 5′ phosphates and 3′ OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.
Características y beneficios
- RNA purification by removing DNA
- Prepare DNA for nick translation1
- Footprinting assays to determine DNA-protein interactions2
Definición de unidad
One unit will produce a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA at pH 5.0 and 25°C
Forma física
Supplied as a solution in 4 mg/ml glycine pH 5.0, 5 mM calcium acetate and 50% glycerol
Nota de preparación
Produced without using any animal cells or animal derived materials.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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