P4069

Phosphatase, Alkaline from Escherichia coli

buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)

• Sinónimos: Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
• Número de CAS: 9001-78-9
• Comisión internacional de enzimas: 3.1.3.1 (BRENDA, IUBMB)
• Número CE: 232-631-4
• Número MDL: MFCD00131849
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: Escherichia coli
• Nivel de calidad: 200
• formulario: buffered aqueous glycerol solution
• actividad específica: 20-50 units/mg protein (in glycine buffer)
• actividad extraña: DNase, RNase, none detected
• temp. de almacenamiento: −20°C

Descripción

Aplicación

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4069 is provided as a buffered aqueous glycerol solution.

Phosphatase, Alkaline from Escherichia coli has been used:

• in fluorometric assay to determine its enzyme activity
• in the dephosphorylating enzyme cocktail for dephosphorylation of peptides
• in treating the nerve and HL60 cell lysate for β-hydroxy β-methylglutaryl-Coenzyme A (HMG-CoA) reductase activity

Acciones bioquímicas o fisiológicas

Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Precaución

Alkaline phosphatase in 5 mM EDTA, pH 8, will be irreversibly heat inactivated at 75°C for 10 minutes.

Definición de unidad

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.

Forma física

Solution in 5 mM Tris-HCl, pH 7.4, 0.5 mM MgCl₂, 0.5 mM ZnCl₂, 50% (w/v) glycerol

Nota de análisis

Protein determined by biuret.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)