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N2770

Sigma-Aldrich

p-Nitrophenyl phosphate

SIGMAFAST™, chromogenic, tablet

Sinónimos:

pNPP tablets

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About This Item

Número CE:
224-246-5
Número MDL:
MFCD00284586
Código UNSPSC:
12352204
NACRES:
NA.83

product name

SIGMAFAST™ p-Nitrophenyl phosphate Tablets, tablet, To prepare 20 mL

formulario

tablet

solubilidad

water: 0.25 tablet/mL, clear, colorless

temp. de almacenamiento

−20°C

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Categorías relacionadas

Aplicación

Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each Sigma FAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.

Reconstitución

Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing pNPP.

Información legal

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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