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GE28-9523-60

GST GraviTrap™

Cytiva 28-9523-60

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About This Item

Código UNSPSC:
41106501
NACRES:
NA.56

ligand

glutathione

envase

pkg of 10 ea

fabricante / nombre comercial

Cytiva 28-9523-60

lecho volumen

2 mL

columna D.I.

15 mm

Matriz

4% agarose

tamaño de partícula

45-165 μm

diámetro promedio

90 μm

cleaning

4-13

Intervalo de trabajo

4-13

capacidad

≥20 mg binding capacity(recombinant glutathione S-transferase (rGST))
 >25 mg binding capacity (horse liver GST/ml medium)

Categorías relacionadas

Descripción general

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
GST GraviTrap™ contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose™ 4B, sufficient for purification of up to 20 mg of fusion protein.

Aplicación

GST GraviTrap™ contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose™ 4B, sufficient for purification of up to 20 mg of fusion Protein. GST-tagged Proteins are recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the Proteins. The binding capacity of prepacked GST GraviTrap™ columns, which can be greater than 10 mg Protein/mL medium, varies with the nature of the tagged Protein and the exact binding conditions used. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged Proteins.

Características y beneficios

  • Convenient, prepacked gravity-fLow columns.
  • Allows direct purification of both clarified and unclarified cell lysates.
  • Yields up to 20 mg of pure Proteins per column.
  • Yields up to 20 mg of pure Proteins per column

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

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Certificados de análisis (COA)

Lot/Batch Number

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Artículos

Troubleshooting Purification Methods

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

Characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.

Troubleshooting Strategies in GST-tagged Protein Purification

This page describes troubleshooting strategies in purification of GST-tagged proteins using GE Healthcare products.

Protocolos

Characteristics of Glutathione Sepharose® Products

Characteristics of Glutathione Sepharose® Products

Global Trade Item Number

Número de referencia del producto (SKU)GTIN
GE28-9523-604061837623646

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