Saltar al contenido
Merck
Todas las fotos(1)

Documentos

GE11-0033-99

His GraviTrap™

Cytiva 11-0033-99

Sinónimos:

His GraviTrap Column

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Código UNSPSC:
12161501
NACRES:
NA.32

envase

pkg of 10 ea

fabricante / nombre comercial

Cytiva 11-0033-99

lecho volumen

1 mL

columna D.I.

15 mm

matriz

6% cross-linked agarose

tamaño de partícula

45-165 μm

diámetro promedio

90 μm

cleaning

2-14(Ni2+-stripped medium)

working range

3-12(Ni2+-stripped medium.)

capacidad

~40 mg binding capacity (histidine-tagged protein/column)(Protein binding capacity is protein-to-protein dependent.)
 ~40 mg binding capacity(histidine-tagged protein)

idoneidad

suitable for bioprocess medium

Categorías relacionadas

Descripción general

His GraviTrap™ is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.

The ready to use column is prepacked with Ni Sepharose™ 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.

His GraviTrap™ columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.

Características y beneficios

  • For fast and simple purification of histidine-tagged* proteins.
  • Convenient, prepacked gravity-flow column.
  • Allows direct purification of both clarified and unclarified cell lysates.
  • High binding capacity; approximately 40 mg of pure protein per column.
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Producto relacionado

Referencia del producto
Descripción
Precios

GE11-0034-00

His Buffer Kit, Cytiva 11-0034-00

Pictogramas

Environment
GHS09

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Artículos

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

Protocolos

Gravity-flow Purification using His GraviTrap™ and His GraviTrap™ Kit

This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from GE Healthcare.

Handling Inclusion Bodies in Recombinant Protein Expression

This page how to isolate proteins from inclusion bodies using GE Healthcare products.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico