GE28-9523-59

GST Spintrap^™

Cytiva 28-9523-59

• Código UNSPSC: 41106500
• NACRES: NA.56

Propiedades

• ligand: glutathione
• envase: pkg of 50 ea
• fabricante / nombre comercial: Cytiva 28-9523-59
• lecho volumen: 50 μL
• matriz: 4% agarose
• tamaño de partícula: 45-165 μm
• diámetro promedio: 90 μm
• cleaning: 4-13
• working range: 4-13
• capacidad: >25 mg binding capacity (horse liver GST/ml medium); ≥500 μg binding capacity(recombinant glutathione S-transferase (rGST))

Descripción

Descripción general

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.

GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates. GST-tagged proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves the functionality of the proteins, and more than 90% purity can be obtained in a single step. GST SpinTrap columns are suitable for purification of multiple samples in parallel, for example in screening experiments such as evaluation of chromatography media, growth temperature, culture density, and other variables to find optimal conditions for expression.

Características y beneficios

• Preserves protein antigenicity and function.
• Yields up to 500 μg of pure protein per column.
• Allows direct purification of both clarified and unclarified cell lysates.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (PBS and 0,05% Kathon CG/ICP Biocide)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

SpinTrap is a trademark of Cytiva

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids