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SAE0045

Sigma-Aldrich

HRV-3C Protease.

N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL

Sinónimos:

Human Rhinovirus 3C Protease, Levlfqgp site protease, PreScission Protease

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About This Item

Código UNSPSC:
12352204
NACRES:
NA.54

origen biológico

human (human Rhinovirus Type 14)

Nivel de calidad

200

Análisis

≥90% (SDS-PAGE)

formulario

aqueous solution

actividad específica

≥5000 U/mg

mol peso

21 kDa

concentración

0.8-1.2 mg/mL

técnicas

protein purification: suitable

idoneidad

suitable for protein modification

aplicaciones

life science and biopharma

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Descripción general

HRV-3C protease from human rhinovirus type 14 is a protease that specifically cleaves within an eight-residue recognition sequence.
Proteolytic cleavage occurs between the Gln and Gly residues.
HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest.
This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography.

Aplicación

HRV-3C Protease has been used to remove the GST tag from the DsCystatin protein.

Definición de unidad

One unit of HRV-3C Protease is defined as the amount of enzyme needed to digest 1nmole of the substrate peptide per hour (H-Glu-Ala-Leu-Phe-Gln-pNA) at 0 °C, in a reaction buffer consisting of 25 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.

Forma física

Supplied in a solution containing 50 mM Tris HCl (pH 7.5), 0.15 M NaCl, 1 mM TCEP and 50% (V/V) glycerol.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Sigma-Aldrich

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