P6181

Endoproteinase Glu-C from Staphylococcus aureus V8

suitable for protein sequencing, lyophilized powder

• Sinónimos: V8 Protease
• Número de CAS: 66676-43-5
• Comisión internacional de enzimas: 3.4.21.19 (BRENDA, IUBMB)
• Número CE: 200-350-6
• Número MDL: MFCD01324998
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• grado: Proteomics Grade
• Nivel de calidad: 200
• formulario: lyophilized powder
• clases químicas de analitos: amino acids
• idoneidad: suitable for protein sequencing
• temp. de almacenamiento: −20°C

Descripción

Aplicación

Endoproteinase Glu-C from Staphylococcus aureus V8 has been used for:

• obtaining proteolytic cleavage fragments of the S-layer protein (from Bacillus stearothermophilus ATCC 12980) to perform affinity studies.
• the enzymatic cleavage of native VSTx-3 (voltage sensor toxin 3) peptide for its sequence determination.
• limited proteolysis of recombinant purified PimA (phosphatidylinositol mannosyltransferase).
• the digestion of glycosylated hemoglobin for isotope dilution liquid chromatography-tandem mass spectrometry analysis.

Acciones bioquímicas o fisiológicas

Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine endoprotease, which hydrolyzes peptide bonds at the carboxyl side of glutamyl and aspartyl residues. The specificity of Glu-C is dependent upon the buffer and pH employed as well as the structure around the potential cleavage site. In ammonium acetate (pH 4.0) or ammonium bicarbonate (pH 7.8), the enzyme preferentially cleaves glutamyl bonds; whereas, in phosphate buffer (pH 7.8) Glu-C will cleave at either site. Glu-C is reported to be active in the presence of 0.2% SDS (sodium dodecyl sulfate) and in 4.0M urea.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H317,H334
• Consejos de prudencia: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Clasificaciones de peligro: Resp. Sens. 1 - Skin Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Faceshields, Gloves