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GE17-5132-01

Glutathione Sepharose™ 4 Fast Flow

Cytiva 17-5132-01, pack of 25 mL

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

glutathione and 10-carbon linker arm

envase

pack of 25 mL

fabricante / nombre comercial

Cytiva 17-5132-01

Matriz

highly cross-linked 4% agarose

diámetro promedio

90 μm

cleaning

3-12

Intervalo de trabajo

3-12

capacidad

>10 mg/mL binding capacity

Categorías relacionadas

Descripción general

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose™ 4 Fast Flow, 25 mL
Glutathione Sepharose™ 4 Fast Flow is an affinity medium for fast, one-step purification of Glutathione S-Transferase (GST) tagged proteins. Provides good flow properties, excellent for scale-up.

Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.

Características y beneficios

  • Fast, one-step purification of GST-tagged proteins
  • Mild elution conditions preserving protein antigenicity and function
  • For scale-up due to high flow properties
  • Glutathione coupled to Sepharose™ 4 Fast Flow via a 10-carbon spacer arm

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

Sepharose is a trademark of Cytiva

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

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Certificados de análisis (COA)

Lot/Batch Number

Lo sentimos, en este momento no disponemos de COAs para este producto en línea.

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Luis J García-Rodríguez et al.
Current biology : CB, 29(9), 1536-1544 (2019-04-23)
For proper chromosome segregation in mitosis, sister kinetochores must interact with microtubules from opposite spindle poles (chromosome bi-orientation) [1, 2]. To promote bi-orientation, Aurora B kinase disrupts aberrant kinetochore-microtubule interactions [3-6]. It has long been debated how Aurora B halts this

Artículos

General Considerations for Purification of GST-tagged Proteins

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

General Considerations for Purification of GST-tagged Protein

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from GE Healthcare.

Protocolos

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

Purification Using GST HiTrap 1 mL and 5 mL Columns: GSTrap 4B, GSTrap FF, and GSTrap HP

This page describes how to purify GST-tagged proteins using GST HiTrap 1 ml and 5 ml columns: GSTrap 4B, GSTrap FF and GSTrap HP from GE Healthcare.

Purification Using GSTrap™ HP, GSTrap™ FF, and GSTrap™ 4B columns

This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.

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