GE17-5282-02
GSTrap™ High Performance
Cytiva 17-5282-02, pack of 5 × 5 mL
About This Item
Productos recomendados
ligand
glutathione
envase
pack of 5 × 5 mL
fabricante / nombre comercial
Cytiva 17-5282-02
Parámetros
<20 mL/min flow rate
42 psi (H2O at room temperature.)
lecho tamaño
16 mm × 25 mm
lecho volumen
5 mL
columna D.I.
16 mm
matriz
highly cross-linked 6% agarose
tam. prom. pieza
34 μm
cleaning
3-12
working range
3-12
capacidad
>7 mg/mL binding capacity (medium)
Categorías relacionadas
Descripción general
GSTrap™ HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose™ High Performance medium, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose™ High Performance. GST-tagged proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer.
GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.
Características y beneficios
- High resolution purification of GST-tagged proteins.
- Mild elution conditions preserving protein antigenicity and function.
- Fast, one-step purification.
- Easy to use with syringe, pump or a chromatography system.
Almacenamiento y estabilidad
Nota de análisis
Otras notas
Información legal
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Código de clase de almacenamiento
3 - Flammable liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from GE Healthcare.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.
This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.
This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.
Protocolos
This page describes how to purify GST-tagged proteins using GST HiTrap 1 ml and 5 ml columns: GSTrap 4B, GSTrap FF and GSTrap HP from GE Healthcare.
This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.
Characteristics of Glutathione Sepharose® Products
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