GE17-5247-01
HisTrap™ High Performance
Cytiva 17-5247-01, pack of 5 × 1 mL
Sinónimos:
Nickel affinity chromatography
About This Item
Productos recomendados
formulario
prepacked column
reticulante
cross-linked
envase
pack of 5 × 1 mL
fabricante / nombre comercial
Cytiva 17-5247-01
condiciones de almacenamiento
(20% Ethanol)
Parámetros
4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature)
42 psi (H2O at room temperature.)
técnicas
liquid chromatography (LC): suitable
lecho tamaño
7 mm × 25 mm
lecho volumen
1 mL
columna D.I.
7 mm
matriz
highly cross-linked 6% agarose
grupo activo de la matriz
Nickel
tam. prom. pieza
34 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
capacidad
≥40 mg binding capacity (histidine-tagged protein/ml medium)(H20 at room temperature)
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
técnica de separación
affinity
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Descripción general
HisTrap™ HP 1 mL and 5 mL columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose™ High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.
HisTrap™ HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags, such as (histidine)4 or (histidine)10. The shorter (histidine)4 will bind more weakly and the longer (histidine)10 will bind more strongly compared with (histidine)6. This difference in binding strength can be used during purification; since (histidine)10 binds more strongly, a higher concentration of imidazole can be added to the lysed cells. This can facilitate the removal of contaminants that can otherwise be copurified with the tagged target protein.
The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.
For convenient scaling up of histidine-tagged protein purification, use 20 mL HisPrep™ FF 16/10 columns. Ni Sepharose™ 6 Fast Flow, the medium prepacked in HisTrap™ FF and HisPrep™ FF 16/10 columns, allows high flow rates, which facilitates scale-up of histidine-tagged protein purification.
Características y beneficios
- High binding capacity, at least 40 mg/mL chromatography medium.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
- Negligible Ni2+ leakage
- Simple manual operation with a syringe, pump, or chromatography system such as AKTA design
Almacenamiento y estabilidad
Nota de análisis
Información legal
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Código de clase de almacenamiento
3 - Flammable liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
This page shows high-throughput screening using His MultiTrap HP and His MultiTrap FF 96-well filter plates from GE Healthcare.
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.
Protocolos
This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from GE Healthcare.
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose High Performance from GE Healthcare.
This page shows how to purify protein A-tagged proteins using GE Healthcare products.
This page how to isolate proteins from inclusion bodies using GE Healthcare products.
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