GE29-0486-84

HiTrap^® Desalting

Cytiva 29-0486-84, pack of 5 mL

• Código UNSPSC: 23151816
• NACRES: NA.56

Propiedades

• envase: pack of 5 mL
• fabricante / nombre comercial: Cytiva 29-0486-84
• disponibilidad: not available in North America
• condiciones de almacenamiento: dry media
• Parámetros: 42 psi
• lecho tamaño: 16 mm × 25 mm
• lecho volumen: 5 mL
• columna D.I.: 16 mm
• matriz: cross-linked dextran
• tamaño de partícula: >20 μm (dry)
• cleaning: 2-13
• working range: 2-13
• idoneidad: suitable for bioprocess medium

Descripción

Descripción general

HiTrap Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.

Aplicación

Gel filtration has successfully been employed for size based separations of macromolecules since the late 1950s. Gel filtration, or size exclusion chromatography, may be performed in three principally different modes, depending on the size differences of the solutes to be separated (i) group separation (ii) fractionation (iii) determination of molecular mass (distributions) When the size difference is large, i.e. a factor of more than 10, we talk about group separation (e.g. desalting, buffer exchange) and when the size difference is small, i.e. a factor of 2 to 5, we talk about fractionation. The third mode is analytical gel filtration, e.g. determination of molecular mass distributions, where an array of molecular masses is to be separated. These different modes put different requirements on the equipment and media used and will yield widely different productivity in terms of information or material processed.

Características y beneficios

• Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series.
• Replaces dialysis as the method of choice for desalting.
• Desalting requires less than one minute due to the high rigidity of Sephadex G-25 and the optimized packing technology.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Otras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Información legal

HiTrap is a registered trademark of Cytiva

Información de seguridad

• Pictogramas: GHS02
• Palabra de señalización: Warning
• Frases de peligro: H226
• Consejos de prudencia: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de clase de almacenamiento: 3 - Flammable liquids