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Key Documents

GE17-0756-04

Glutathione Sepharose 4B

Cytiva 17-0756-04, pack of 300 mL

Sinónimos:

Glutathione Column

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

glutathione

envase

pack of 300 mL

fabricante / nombre comercial

Cytiva 17-0756-04

matriz

4% agarose

tamaño de partícula

45-165 μm

diámetro promedio

90 μm

cleaning in place

4-13

working range

4-13

capacidad

>25 mg binding capacity (horse liver GST/ml medium)

Descripción general

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

Características y beneficios

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palabra de señalización

Warning

Frases de peligro

Código de clase de almacenamiento

3 - Flammable liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

This page addresses common problems associated with the expression and growth of GST-tagged proteins using GE Healthcare products.

This page shows how to purify GST-tagged proteins using GST SpinTrap from GE Healthcare.

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

Ver todo

Protocolos

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from GE Healthcare.

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.

This page describes how to purify GST-tagged proteins using GST HiTrap 1 ml and 5 ml columns: GSTrap 4B, GSTrap FF and GSTrap HP from GE Healthcare.

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.

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