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Merck
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B6429

Sigma-Aldrich

Casein Blocking Buffer 10x

for Northern and Southern blotting, solution

Sinónimos:

10x Blocking Buffer

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About This Item

Código UNSPSC:
12161700
NACRES:
NA.54

grado

Molecular Biology
for molecular biology

Formulario

solution

técnicas

Southern blotting: suitable

temp. de almacenamiento

room temp

Categorías relacionadas

Descripción general

Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.

Aplicación

Casein Blocking Buffer 10x has been used in:

  • western blotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunohistochemistry

Características y beneficios

  • Better blocking performance than dried milk or BSA
  • Easy-to-use concentrate
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent and colorimetric detection assays

Nota de preparación

Dilute 10x Blocking Buffer with molecular biology grade water before use.

Opcional

Referencia del producto
Descripción
Precios

Pictogramas

Exclamation markEnvironment
GHS07,GHS09

Palabra de señalización

Warning

Frases de peligro

H315,H317,H319,H411

Clasificaciones de peligro

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
SLCM2272
SLCL0388
SLCK0571
SLCJ1724
SLCF9112

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Niculescu MD, et al.
Journal of Neurochemistry, 89(5), 1252-1259 (2004)
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Fish collagen is widely used in medicine, cosmetics, and the food industry. However, its clinical relevance as an allergen is not fully appreciated. This is likely due to collagen insolubility in neutral aqueous solutions, leading to low abundance in commercially
Mihai D Niculescu et al.
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The availability of choline during critical periods of fetal development alters hippocampal development and affects memory function throughout life. Choline deficiency during fetal development reduces proliferation and migration of neuronal precursor cells in the mouse fetal hippocampus and these changes
Michelle M Adams et al.
Experimental neurology, 211(1), 141-149 (2008-03-18)
Caloric restriction (CR) is a daily reduction of total caloric intake without a decrease in micronutrients or disproportionate reduction of any one dietary component. CR can increase lifespan reliably in a wide range of species and appears to counteract some
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Protocolos

10x Blocking Buffer Protocol

Membrane-based blotting applications that employ enzyme conjugates to generate colorimetric or chemiluminescent signal require the use of an added blocking step to decrease the signal generated by non-specific binding.

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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