T9285

Tampón Tris-EDTA

100X concentrate for Northern and Southern blotting

• Sinónimos: Tampón TE solution
• Número MDL: MFCD00236359
• Código UNSPSC: 41105319
• ID de la sustancia en PubChem: 329826904
• NACRES: NA.25

Propiedades

• grado: for molecular biology
• Nivel de calidad: 200
• esterilidad: 0.2 μm filtered
• formulario: liquid
• pH: 7.8-8.2 (1 × in water)
• aplicaciones: sample preparation
• actividad extraña: DNase, RNase, none detected
• temp. de almacenamiento: room temp
• cadena SMILES: NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
• Clave InChI: VLEIUWBSEKKKFX-UHFFFAOYSA-N

Descripción

Descripción general

Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.

Aplicación

TE buffer has been used for:

• isolate, resuspend, or store DNA and RNA for PCR or qPCR
• gel electrophoresis, including Northern and Southern blotting
• bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells
• antigen retrieval for immunohistochemistry

Componentes

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Otras notas

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Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable