T9650

Tris Acetate-EDTA buffer

10× concentrate, BioReagent, for molecular biology, non-sterile; 0.2 μm filtered

• Sinónimos: TAE buffer
• Número MDL: MFCD00236357
• Código UNSPSC: 41105319
• ID de la sustancia en PubChem: 329826908
• NACRES: NA.25

Propiedades

• grado: for molecular biology
• Nivel de calidad: 200
• esterilidad: non-sterile; 0.2 μm filtered
• Línea del producto: BioReagent
• formulario: solution
• idoneidad: suitable for gel electrophoresis (after dilution to working concentration)
• actividad extraña: Protease, none detected; RNAse, none detected
• cadena SMILES: CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
• Clave InChI: HGEVZDLYZYVYHD-UHFFFAOYSA-N

Descripción

Aplicación

Tris Acetate-EDTA buffer has been used for the preparation of agarose gel during DNA agarose gel electrophoresis.

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Otras notas

0.4 M Tris acetate, pH approx. 8.3, containing 0.01 M EDTA.

Nota de preparación

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Solution prepared with 18 megohm water

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Warning
• Frases de peligro: H373
• Consejos de prudencia: P260 - P313 - P501
• Clasificaciones de peligro: STOT RE 2 Inhalation
• Órganos de actuación: Respiratory Tract
• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable