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Merck

G7663

Sigma-Aldrich

Gelatin blocking buffer

for Western blotting, powder blend

Sinónimos:

blocking buffer

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About This Item

Código UNSPSC:
41105300
NACRES:
NA.25

grado

for molecular biology

Nivel de calidad

Formulario

powder blend

temp. de almacenamiento

room temp

Descripción general

Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).

Aplicación

Suitable for use as a non-specific blocking agent for Western blots.

Reconstitución

Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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A Fortin et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 72(5-6), 239-243 (1994-05-01)
The major advantages of the horseradish peroxidase chemiluminescence (HRP-CL) immunodetection method in Western blot analysis are its high sensitivity, nonradioactive detection, economy of the primary antibody, and speed of detecting the signal. However, we observed a strong and reproducible signal
K A Brogden et al.
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A sensitive cell-based assay for the detection of residual infectious West Nile virus.
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Enterotoxigenic Escherichia coli (ETEC) strains are leading causes of childhood diarrhea in developing countries. Adhesion is the first step in pathogenesis of ETEC infections and ETEC pili designated colonization factor antigens (CFAs) are believed to be important in the biofim

Protocolos

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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