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C7594

Sigma-Aldrich

Casein Blocking Buffer

for Northern and Southern blotting, powder blend

Sinónimos:

blocking buffer

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About This Item

Número CE:
232-555-1
Código UNSPSC:
12161700
NACRES:
NA.25

grado

for molecular biology

Nivel de calidad

200

formulario

powder blend

técnicas

Southern blotting: suitable

pH

6.8-7.2(reconstituted per directions)

solubilidad

H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)

temp. de almacenamiento

room temp

Categorías relacionadas

Descripción general

Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.

Aplicación

Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.

Características y beneficios

  • Better blocking performance than dried milk or BSA
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent, colorimetric , and DIG detection assays

Nota de preparación

Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.

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Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protocolos

Casein Blocking Buffer for Southern or Western Blotting

Unoccupied sites on the solid support must be blocked to detect an immobilized antigen. This protocol describes how to use our Casein Blocking Buffer in Southern and Western blotting applications, including options for fluorescein and DIG detection systems.

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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