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PP0100

Sigma-Aldrich

Trypsin-Profil IGD-Kit

Synonym(e):

In gel digestion kit, Proteomics grade Trypsin, TPCK treated

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.56

Verwendung

 kit sufficient for ≤100 applications

Qualitätsniveau

200

Lagertemp.

2-8°C

Allgemeine Beschreibung

The Trypsin Profile IGD Kit enables fast, efficient, and complete in-gel tryptic digestion of up to 100 excised protein spots. Digested proteins are ready for MALDI-MS (matrix-assisted laser desorption/ionization-mass spectrometry) and require no additional preparation. Because the Trypsin Profile IGD Kit contains Proteomics Grade Trypsin, a higher sequence coverage and fewer ambiguous autolytic peaks are observed in MALDI spectra.
Trypsin has been chemically modified through reductive methylation of the ε-amino groups of lysine to reduce autolysis and minimize autolytic fragments. In addition, it has been TPCK (tosyl phenylalanyl chloromethyl ketone) treated to remove residual chymotrypsin activity and then further purified by affinity chromatography, yielding a highly purified trypsin suitable for proteomics work.

Anwendung

Trypsin Profile IGD Kit has been used for in-gel digestion for LC-MS (liquid chromatography-mass spectrometry)/MS and MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry analysis.

Leistungsmerkmale und Vorteile

  • Schnelleres Entfärben als mit alternativen In-Gel Verdau-Kits
  • Enthält 100% der Reagenzien für Entfärbung, Verdau und Extraktion der Proteine?/Peptide von Interesse nötig sind
  • Kompatibel mit Coomassie, SYPRO® Orange, SYPRO Ruby, und ProteoSilver™ gefärbten Gelen
  • Die resultierenden Proben sind bereit für die Analyse durch MALDI-MS oder HPLC-MS

Rechtliche Hinweise

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
SYPRO is a registered trademark of Life Technologies

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung
  • Destaining Solution Reconstituted to 75

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

3 - Flammable liquids

Flammpunkt (°F)

35.6 °F - closed cup

Flammpunkt (°C)

2.0 °C - closed cup

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Caroline L Weldon et al.
Toxicon : official journal of the International Society on Toxinology, 55(2-3), 558-569 (2009-10-20)
The Puerto Rican Racer Alsophis portoricensis is known to use venom to subdue lizard prey, and extensive damage to specific lizard body tissues has been well documented. The toxicity and biochemistry of the venom, however, has not been explored extensively.
Identification of plant viruses using one-dimensional gel electrophoresis and peptide mass fingerprints.
Luo, H., et al.
J. Virol. Methods, 165(2), 297-301 (2010)
SILAM for quantitative proteomics of liver Akt1/PKBa after burn injury.
Lu XM, et al.
International Journal of Molecular Medicine, 29, 461-461 (2012)
Shuo Han et al.
Cell death & disease, 12(11), 1070-1070 (2021-11-12)
Uncontrolled overactivation of autophagy may lead to autophagic cell death, suppression of which is a pro-survival strategy for tumors. However, mechanisms involving key regulators in modulating autophagic cell death remain poorly defined. Here, we report a novel long noncoding RNA
X-M Lu et al.
International journal of burns and trauma, 3(1), 37-48 (2013-02-07)
Insulin resistance is a major effect of burn injury and insulin receptor substrate-1 (IRS-1) plays an important role in signal transduction. Here, we explored the integrity of IRS-1 in muscle after burn injury. A murine model of severe burn injury
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Post Translational Modification

Post-translational modifications such as glycosylation, phosphorylation, and sulfation, to name a few, serve many functions. As a result, the analysis of proteins and their post-translational modifications is particularly important for the study of diseases where multiple genes are known to be involved, such as heart disease, cancer and diabetes.

Ultra-Pure MALDI Matrices

One of the most important aspects of our ultra-pure MALDI matrix substances is their ability to dissolve rapidly and completely; a brief vortex mixing is typically sufficient.

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