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T6424

Sigma-Aldrich

Trypsin aus meschlichem Pankreas

salt-free, lyophilized powder, vial of ≥1,000 BAEE units

Synonym(e):

Serine Protease 1

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About This Item

CAS-Nummer:
9002-07-7
EC-Nummer:
3.4.21.4 (BRENDA, IUBMB)
EG-Nummer:
232-650-8
MDL-Nummer:
MFCD01323069
UNSPSC-Code:
12352204
NACRES:
NA.54

Biologische Quelle

human pancreas

Qualitätsniveau

200

Form

salt-free, lyophilized powder

Verpackung

vial of ≥1,000 BAEE units

UniProt-Hinterlegungsnummer

P07477
P07478
P35030

Lagertemp.

−20°C

Angaben zum Gen

human ... PRSS1(5644) , PRSS2(5645) , PRSS3(5646)

Allgemeine Beschreibung

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Trypsin is a serine protease. The pancreas produce the precursor trypsinogen and secretes it to the intestine. Trypsinogen is converted to trypsin by enterokinase in the intestinal mucous membrane.

Anwendung

Trypsin has been used in a study to assess the similarities between the hepatitis E virus and human astrovirus. Trypsin has also been used in a study to characterize a unique technique for culturing primary adult human epithelial progenitor, or stem, cells.

Einheitendefinition

One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

Menge

Minimum 1,000 BAEE units/vial

Hinweis zur Analyse

Proteingehalt mittels E1%/280.

Sonstige Hinweise

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer

Inhibitor

Produkt-Nr.
Beschreibung
Preisangaben

B6506

Benzamidin -hydrochlorid Hydrat, ≥99%

T9003

Trypsin-Inhibitor aus Glycine max (soybean), lyophilized powder

M6159

α2-Macroglobulin aus Humanplasma, BioUltra, ≥98% (SDS-PAGE)

D7910

3,4-Dichlorisocumarin, serine protease inhibitor

D0879

Diisopropylfluorphosphat

L2023

Leupeptin trifluoroacetate salt, ≥90% (HPLC), microbial

A6191

Antipain -dihydrochlorid aus microbieller Quelle, protease inhibitor

T9777

Trypsin-chymotrypsin inhibitor from Glycine max (soybean), lyophilized powder

A1153

Aprotinin aus Rinderlunge, lyophilized powder, 3-8 TIU/mg solid

P7626

Phenylmethansulfonylfluorid, ≥98.5% (GC)

A9024

α1-Antitrypsin aus Humanplasma, salt-free, lyophilized powder

A6150

α1-Antitrypsin aus Humanplasma, salt-free, lyophilized powder

T2011

Trypsin-Inhibitor aus Hühnereiweiss, Type III-O (free of ovoinhibitor)

Substrat

Produkt-Nr.
Beschreibung
Preisangaben

Piktogramme

Health hazardExclamation mark
GHS08,GHS07

Signalwort

Danger

Gefahreneinstufungen

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Industrial Enzymes and Their Applications (1998)
Kelly A Dryden et al.
Journal of molecular biology, 422(5), 650-658 (2012-06-30)
Human astroviruses (HAstVs) are a major cause of gastroenteritis. HAstV assembles from the structural protein VP90 and undergoes a cascade of proteolytic cleavages. Cleavage to VP70 is required for release of immature particles from cells, and subsequent cleavage by trypsin
Jia Liu et al.
Nature protocols, 16(7), 3522-3546 (2021-06-06)
Cellular heterogeneity is pervasive and of paramount importance in biology. Single-cell analysis techniques are indispensable for understanding the heterogeneity and functions of cells. Low-copy-number proteins (fewer than 1,000 molecules per cell) perform multiple crucial functions such as gene expression, cellular
Cynthia Luz Marcelo et al.
BMC dermatology, 12, 8-8 (2012-06-26)
Primary keratinocytes derived from epidermis, oral mucosa, and urothelium are used in construction of cell based wound healing devices and in regenerative medicine. This study presents in vitro technology that rapidly expands keratinocytes in culture by growing monolayers under large
Olivier Rivoire
Physical review letters, 110(17), 178102-178102 (2013-05-18)
Studies of coevolution of amino acids within and between proteins have revealed two types of coevolving units: coevolving contacts, which are pairs of amino acids distant along the sequence but in contact in the three-dimensional structure, and sectors, which are
Alle zugehörigen Dokumente anzeigen

Protokolle

Procedure for Enzymatic Assay of Trypsin (EC 3.4.21.4)

This procedure is for products with a specification for Trypsin activity using Na-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determination (A253, Light path = 1 cm).

Verwandter Inhalt

Trypsin Serine Protease Enzyme

Trypsin is an enzyme in the serine protease class that consists of a polypeptide chain of 223 amino acid residues. Multiple sources, grades and formulations of trypsin specifically designed for research applications are available.

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