U4002
Urease from Canavalia ensiformis (Jack bean)
Type IX, powder, 50,000-100,000 units/g solid
Sinonimo/i:
Jack bean urease, Urea amidohydrolase
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About This Item
Prodotti consigliati
Tipo
Type IX
Livello qualitativo
Stato
powder
Attività specifica
50,000-100,000 units/g solid
PM
~544620 Da
Temperatura di conservazione
2-8°C
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Descrizione generale
Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Composed of six subunits with total molecular weight: ~544,620
Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease binds to nickel and is present in archaea, bacteria, unicellular eukaryotes and plants. Jack bean urease was the first enzyme to be crystallized and the first enzyme found to contain nickel. It is a multi-subunit enzyme, consisting of 91 kDa subunits in three protein forms.
Applicazioni
Urease from Canavalia ensiformis (Jack bean) has been used: to spike urine samples to determine the hydrolysis rate of urea in pure urine It has also been used to study the inhibitory action of polyhalogenated benzo- and naphthoquinones on Bacillus pasteurii and Canavalia ensiformis.
Azioni biochim/fisiol
In Canavalia ensiformis (Jack Bean), urease facilitates the conversion of L-canavanine to canaline.
Urease is involved in purine metabolism and the urea cycle. It hydrolyze urea to produce ammonia and carbon dioxide.
Definizione di unità
One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
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I clienti hanno visto anche
G A Rosenthal et al.
Plant physiology, 76(2), 541-544 (1984-10-01)
l-Canavanine, the guanidinooxy structural analog of l-arginine, is an important nonprotein amino acid of many leguminous plants with nitrogen storage a major proported role. l-[Guanidinooxy-(14)C]canavanine, [(14)C] urea, and [(15)N]urea were injected separately into the fleshy, green cotyledons of 9-day old
Armored Urease: Enzyme-Bioconjugated Poly(acrylamide) Hydrogel as a Storage and Sensing Platform
Kunduru KR, et al.
Test, 590, 143-167 (2017)
An investigation of the use of urease-antibody conjugates in enzyme immunoassays
Chandler HM, et al.
Journal of Immunological Methods, 53(2), 187-194 (1982)
Yersinia enterocolitica
Molecular Medical Microbiology, 1319-1344 (2015)
In-toilet disinfection of fresh fecal sludge with ammonia naturally present in excreta
Ogunyoku TA, et al.
Journal of Water, Sanitation and Hygiene for Development, 6(1), 104-114 (2016)
Articoli
probiotics-and-human
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