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U1500

Sigma-Aldrich

Urease from Canavalia ensiformis (Jack bean)

Type III, powder, 15,000-50,000 units/g solid

Sinonimo/i:

Jack bean urease, Urea amidohydrolase

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About This Item

Numero CAS:
9002-13-5
Classificazione EC (Enzyme Commission):
3.5.1.5 (BRENDA, IUBMB)
Numero CE:
232-656-0
Numero MDL:
MFCD00070858
Codice UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54

Origine biologica

Canavalia ensiformis

Livello qualitativo

300

Tipo

Type III

Forma fisica

powder

Attività specifica

15,000-50,000 units/g solid

PM

~544620 Da

applicazioni

diagnostic assay manufacturing

Temperatura di conservazione

2-8°C

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Descrizione generale

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease is a cytosolic enzyme that catalyzes the hydrolysis of urea to ammonia and carbon dioxide, with initial conversion to carbamate, and the carbamate breaks down further, on hydrolysis, into ammonia and to carbonic acid / carbonate. Urease is a metalloenzyme that contains nickel as a co-factor. Urease occurs naturally in plants, fungi, and certain bacteria. James B. Sumner first isolated urease from jack bean in 1926.

Azioni biochim/fisiol

Urease from Canavalia ensiformis functions as an insecticide by the release of a toxic small peptide cleaved by proteases found in the gut of insects.

Definizione di unità

One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

Pittogrammi

Health hazardExclamation mark
GHS08,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H315,H319,H334,H335

Classi di pericolo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Journal of insect physiology, 55(3), 255-263 (2009-01-06)
Ureases are metalloenzymes that are widespread among plants, fungi and bacteria. Urease isoforms (jack bean urease-JBU and canatoxin) from Canavalia ensiformis seeds are toxic to insects and fungi, suggesting a role in plant defense. The entomotoxic effect is due to
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