94280
Urease from Canavalia ensiformis (Jack bean)
powder, ~1 U/mg
Sinonimo/i:
Jack bean urease, Urea amidohydrolase
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Prodotti consigliati
Stato
powder
Livello qualitativo
Attività specifica
~1 U/mg
PM
Mr ~480000
Temperatura di conservazione
−20°C
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
Descrizione generale
Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Composed of six subunits with total molecular weight: ~544,620
Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease consists of nickel and is present in archaea, bacteria, unicellular eukaryotes and plants.
Applicazioni
Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes . It may be useful for the detection of pathogens as well as heavy-metal ions.
Urease from Canavalia ensiformis (Jack bean) has been used in purification and crystallization studies.
Azioni biochim/fisiol
Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit .
Definizione di unità
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol urea (Cat. No. 51459) per minute at pH 8.0 and 25 °C.
Altre note
Application in (selective) hydrolysis/condensations; structure.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Dispositivi di protezione individuale
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Scegli una delle versioni più recenti:
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
![(S,S)-(+)-2,6-Bis[2-(hydroxydiphenylmethyl)-1-pyrrolidinyl-methyl]-4-methylphenol 95%](/deepweb/assets/sigmaaldrich/product/structures/126/939/bff1a61c-8335-434f-8da4-9b1929aef17f/640/bff1a61c-8335-434f-8da4-9b1929aef17f.png)
K Takishima et al.
European journal of biochemistry, 175(1), 151-165 (1988-07-15)
The amino acid sequence of jack bean urease has been determined. The protein consists of a single kind of polypeptide chain containing 840 amino acid residues. The subunit relative molecular mass calculated from the sequence is 90,770, indicating that urease
M J Sculley et al.
Biophysical chemistry, 19(1), 39-47 (1984-01-01)
The use of bifunctional reagents to form cross-links between subunits in protein oligomers and subsequent disruption of noncovalent interactions with SDS allows comment upon the number of subunits and the symmetry in the original assembly. In existing treatments the number
Isothermal titration calorimetry to characterize enzymatic reactions
Methods in Enzymology, 567, 215-236 (2016)
I.A. Natchev
Tetrahedron, 44, 1511-1511 (1988)
M.C.N. Jayasuriya et al.
Anim. Feed Sci. Technol., 8, 271-271 (1983)
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.