Passa al contenuto
Merck
Tutte le immagini(1)

Key Documents

SRP6450

Sigma-Aldrich

Neprilysin/CD10 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Sinonimo/i:

CALLA, DKFZp686O16152, MGC126681, MGC126707, MME, NEP, SFE

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12352200
NACRES:
NA.32

Origine biologica

human

Ricombinante

expressed in HEK 293 cells

Saggio

≥95% (SDS-PAGE)

Forma fisica

lyophilized

PM

calculated mol wt 80 kDa
observed mol wt 90-100 kDa (DTT-reduced. Tyr 52 is the predicted N-terminal .)

Confezionamento

pkg of 10 μg

Impurezze

<1 EU/μg endotoxin (LAL test)

N° accesso UniProt

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... MME(4311)

Descrizione generale

Cluster of differentiation 10 (CD10) is also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), neprilysin and common acute lymphoblastic leukemia antigen (CALLA). It is a 90-110kDa type II transmembrane glycoprotein normally expressed by a variety of tissues, including epithelial cells of the prostate, kidney, intestine, endometrium, adrenal glands and lung. The protein has a cytoplasmic amino-terminal domain, a transmembrane hydrophobic domain, and an extracellular domain which possesses catalytic activity. The gene encoding this protein is localized on human chromosome 3q25.2.

Azioni biochim/fisiol

Cluster of differentiation 10 (CD10) is a zinc-dependent metalloprotease enzyme that cleaves peptide bonds on the amino side of hydrophobic residues and inactivates a variety of physiologically active secreted peptides. It is thought to be the rate-limiting degrading enzyme of amyloid-β peptide (Aβ) whose abnormal misfolding and aggregation in neural tissue has been implicated in the development of Alzheimer′s disease (AD). CD10 is also identified as the common acute lymphoblastic leukemia antigen (CALLA) present on leukemic cells of pre-B phenotype, and thus serves as the most important biomarker in the diagnosis of human acute lymphocytic leukemia (ALL).

Stato fisico

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 150 mM NaCl, pH 8.0 Normally Mannitol or Trehalose is added as protectants before lyophilization.

Ricostituzione

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Mari van de Vyver et al.
Stem cells and development, 30(23), 1141-1152 (2021-06-17)
Monitoring wound progression over time is a critical aspect for studies focused on in-depth molecular analysis or on evaluating the efficacy of potential novel therapies. Histopathological analysis of wound biopsies can provide significant insight into healing dynamics, yet there is
Role of B Cell Development Marker CD10 in Cancer Progression and Prognosis.
Mishra D
Molecular Biology International, 4328697-4328697 (2016)

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.