C8696

Cathepsin D from human liver

lyophilized powder, ≥250 units/mg protein (E1%/280)

• Numero CAS: 9025-26-7
• Classificazione EC (Enzyme Commission): 3.4.23.5 (BRENDA, IUBMB)
• Numero MDL: MFCD00130749
• Codice UNSPSC: 12352204
• NACRES: NA.32

Proprietà

• Forma fisica: lyophilized powder
• Livello qualitativo: 200
• Attività specifica: ≥250 units/mg protein (E1%/280)
• PM: ~45 kDa
• Colore: white
• N° accesso UniProt: P07339
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... CTSD(1509)

Descrizione

Descrizione generale

Cathepsin D is an aspartic protease, which is located in lysosomes. It is involved in protein catabolism and maintains hormone and antigen processing. Cathepsin D is implicated in neoplasia and neurodegenerative changes. It regulates lysosomal proteolysis and endogenous fibrinolysis.

Applicazioni

Cathepsin D from human liver has been used:

• in β-secretase activity assay
• for enzymatic degradation of amyloid β 1-42
• in microinjection of human foreskin fibroblasts

Azioni biochim/fisiol

Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.

Altre note

Contains α, β and γ isoenzymes as observed by isoelectric-focusing.

Definizione di unità

One unit will produce an increase in A₂₈₀ of 1.0 in 30 min at pH 3.3 at 37 °C measured as TCA-soluble products using acid-denatured hemoglobin as substrate (1 cm light path).

Stato fisico

Powder containing sodium phosphate buffer salt.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable