SRP3138

PDGF-BB human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Sinonimo/i: Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF), Platelet-Derived Growth Factor-BB
• Codice UNSPSC: 12352202
• eCl@ss: 32190102
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in E. coli
• Saggio: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Forma fisica: lyophilized
• Potenza: 1.0-3.0 ng/mL ED₅₀
• PM: 24.3 kDa
• Confezionamento: pkg of 10 μg
• tecniche: cell culture | mammalian: suitable
• Impurezze: <0.1 EU/μg endotoxin, tested
• Colore: white to off-white
• N° accesso UniProt: P01127
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... PDGFB(5155)

Descrizione

Descrizione generale

PDGFs (platelet-derived growth factors) are disulfide-linked dimers consisting of two 12.0-13.5kDa polypeptide chains, designated PDGF-A and PDGF-B chains. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. The gene PDGF-B is mapped to human chromosome 22q13.1. Recombinant human PDGF-BB is a 24.3kDa disulfide-linked homodimer of two B chains (218 total amino acids).

Applicazioni

PDGF-BB human has been used in the cell viability assay. It has been used as a stimulator in PDGFRα (platelet-derived growth factor receptor α)-positive HTPCs (human testicular peritubular cells).

Azioni biochim/fisiol

The three naturally occurring PDGFs (platelet-derived growth factors); PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGF-B along with PDGFR-β participates in vascular maturation. It brings smooth muscle cells and pericytes to growing vessels. Heterozygous mutation in PDGF-B is associated with primary familial brain calcification.

Sequenza

SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT

Stato fisico

Lyophilized from 10 mM Acetic Acid.

Ricostituzione

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H315,H319
• Consigli di prudenza: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Classi di pericolo: Eye Irrit. 2 - Skin Irrit. 2
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable