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SAB4200770

Sigma-Aldrich

Anti-Human IgG4-Peroxidase antibody, Mouse monoclonal

clone HP-6025, purified from hybridoma cell culture

Sinonimo/i:

Anti-Human immunoglobulin G4

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

mouse

Livello qualitativo

200

Coniugato

peroxidase conjugate

Forma dell’anticorpo

purified from hybridoma cell culture

Tipo di anticorpo

primary antibodies

Clone

HP-6025, monoclonal

Forma fisica

lyophilized powder

Reattività contro le specie

human

Concentrazione

~2 mg/mL

tecniche

ELISA: 1:80,000-1:160,000 using 1 μg/mL human IgG4 for coating

Isotipo

IgG1

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Human IgG consists of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. It constitutes approximately 65, 30, 5 and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens and pathological conditions. Only IgG1 and IgG3 are capable of adhering to mononuclear phagocytes while IgG2 and IgG4 auto-antibodies are not associated with disorders such as hemolytic anemia.Serum IgG subclass deficiencies have been recorded for different patient groups. For example, IgG2 and IgG4 deficiency is associated with IgA deficiency as found in patients of ataxia telangiextasia. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and thus may assist in the diagnosis of various disorders. For example IgG subclass expression pattern in malaria patients reveals positive association of IgG4 levels and malaria antigens but not with patients age.Monoclonal Anti-Human IgG4 specifically recognizes Human IgG4. The antibody shows no cross-reactivity with human IgG1, IgG2 and IgG3. The IUIS/WHO study evlauted this monoclonal antibody as one of the most widely applicable IgG4 specific monoclonal antibodies

Immunogeno

Purified human IgG4 myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads

Applicazioni

The antibody is recommended to use in various immunological techniques, including ELISA.

Stato fisico

Supplied as lyophilized powder. After reconstitution with 0.25 mL of distilled water to a final antibody concentration of ∼ 2 mg/mL, the solution contains 1% BSA, 2.5% trehalose, 0.05% MIT in 0.01 M sodium phosphate buffered saline

Altre note

Reconstitute the content of the vial with 0.25 ml of distilled water to a final antibody concentration of ∼2 mg/ml.

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Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H317

Classi di pericolo

Skin Sens. 1

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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P Aucouturier et al.
Clinical and experimental immunology, 68(2), 392-396 (1987-05-01)
Serum IgG subclass levels were determined using a competitive indirect immunoenzymatic assay with monoclonal antibodies in 16 patients with ataxia-telangiectasia. Eight children had IgA deficiency, two had IgG and IgA deficiency and six patients showed no immunoglobulin class abnormality. However
A Nasr et al.
Scandinavian journal of immunology, 74(4), 390-396 (2011-06-08)
The anti-malarial IgG immune response during the lengthy and dry season in areas of low malaria transmission as in Eastern Sudan is largely unknown. In this study, ELISA was used for the measurement of pre-existing total IgG and IgG subclasses
J D Isaacs et al.
Clinical and experimental immunology, 106(3), 427-433 (1996-12-01)
It is traditionally held that human IgG4 MoAbs should not deplete target cells in vivo, as this isotype is inactive in a number of in vitro assays that measure effector function. We have previously challenged this dogma, and the current
F W van der Meulen et al.
British journal of haematology, 46(1), 47-56 (1980-09-01)
The purpose of this study was to determine whether quantitative or qualitative factors are of major importance in the destruction of red cells sensitized with incomplete warm autoantibodies of subclass IgG1. To that end, the relative amount of igG1 antibody
R Jefferis et al.
Immunology letters, 10(3-4), 223-252 (1985-01-01)
Seventy-four monoclonal antibodies (McAb) of putative specificity for human IgG (11), the IgG sub-classes (59) or Gm allotypes (4) have been evaluated for reactivity and specificity in eight laboratories employing different assay techniques or protocols. For the IgG, IgG3, IgG4
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