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S8761

Sigma-Aldrich

Sodium Bicarbonate

Solution (7.5%), sterile-filtered, suitable for cell culture

Sinonimo/i:

aqueous sodium bicarbonate

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About This Item

Numero CAS:
Numero MDL:
Codice UNSPSC:
12161702
ID PubChem:
NACRES:
NA.75

product name

Sodium bicarbonate solution, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture

Sterilità

sterile-filtered

Nome Commerciale

BioReagent

Forma fisica

solution (7.5%)

tecniche

cell culture | mammalian: suitable

Impurezze

endotoxin, tested

pH

7.8-8.2

Temperatura di conservazione

2-8°C

Stringa SMILE

[Na+].OC([O-])=O

InChI

1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
UIIMBOGNXHQVGW-UHFFFAOYSA-M

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Categorie correlate

Descrizione generale

Sodium bicarbonate (NaHCo3) is a commonly used alkaline buffering agent(5) in laboratories. The addition of this buffer to the cell culture medium aids in maintaining the appropriate pH by using the carbon dioxide present in the incubator. In addition to its buffering properties, Sodium bicarbonate also acts as a source of sodium and aids in sodium gluconate synthesis during the fermentation process.

Applicazioni

Sodium bicarbonate solution has been used:
  • as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice
  • as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants
  • as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma
  • to prepare collagen mix droplets for 3D collagen sprouting assay
  • to prepare collagen matrix for rat epidermal keratinocyte (REK) organotypic culture

Nota sulla preparazione

Prepared in cell culture grade water.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Protocolli

3-D Culture Matrix™ Collagen I may be used as a gel on which to grow cells or a media additive alone or in concert with other basement membrane components to study cellular growth and differentiation in three dimensions in vitro.

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