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D2429

Sigma-Aldrich

DMEM - low glucose

With sodium pyruvate, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable fore cell culture

Sinonimo/i:

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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About This Item

Numero MDL:
MFCD00217342
Codice UNSPSC:
12352207
NACRES:
NA.75

Nome del prodotto

Dulbecco′s Modified Eagle′s Medium - low glucose, 10 ×, With 1000 mg/L glucose (1x), without L-glutamine, sodium bicarbonate, and folic acid, liquid, sterile-filtered, suitable for cell culture

Sterilità

sterile-filtered

Stato

liquid

Concentrazione

10 ×

tecniche

cell culture | mammalian: suitable

Impurezze

endotoxin, tested

Componenti

NaHCO3: no
sodium pyruvate: yes
phenol red: yes
glucose: low
L-glutamine: no
HEPES: no

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Categorie correlate

Descrizione generale

Dulbecco′s Modified Eagle′s Medium (DME) is a modification of basal medium Eagle (BME) that contains a 4-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose.

Applicazioni

Dulbecco′s Modified Eagle′s Medium-low glucose has been used:
  • during organoid embedding for neutralizing thawed collagen I
  • to culture melanoma cancer cell lines
  • to store the cancer tissue samples
  • as a differentiation culture medium for C2C12 cells

Ricostituzione

Supplement with 3.7 g/L sodium bicarbonate, 0.584 g/L L-glutamine, 0.004 g/L folic acid, at 1×.

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Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H317

Classi di pericolo

Skin Sens. 1

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
RNBL7902
RNBK8505
RNBJ9318
RNBJ4442
RNBJ2213

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Maria Guix et al.
Scientific reports, 6, 21701-21701 (2016-02-26)
Novel approaches to develop naturally-induced drug delivery in tumor environments in a deterministic and controlled manner have become of growing interest in recent years. Different polymeric-based microstructures and other biocompatible substances have been studied taking advantage of lactic acidosis phenomena
Yi Li et al.
Physiological genomics, 50(3), 158-168 (2018-01-18)
Folic acid supplements taken during pregnancy can prevent neural tube defects and other developmental abnormalities. Here, we explored the effects of folate supplementation on gene expression and DNA methylation during C2C12 differentiation. Based on the folic acid concentration, this study
Tejas Shah et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 84, 526-534 (2016-10-04)
Folic acid (FA) and iron are essential supplements during pregnancy. Similarly effects of vitamin B12 (B12) inadequacy and high folate and low B12 status, on pregnancy outcome are available. However there are no mandatory recommendations for B12. There are many
Qinjie Weng et al.
Scientific reports, 7(1), 1705-1705 (2017-05-13)
Folate, an essential micronutrient, is a critical cofactor in one-carbon metabolism for many cellular pathways including DNA synthesis, metabolism and maintenance. Folate deficiency has been associated with an increased risk of neurological disease, cancer and cognitive dysfunction. Dihydrofolate reductase (DHFR)
Antonietta Aversano et al.
International journal of molecular medicine, 46(4), 1359-1366 (2020-09-19)
Balneotherapy and spa therapy have been used in the treatment of ailments since time immemorial. Moreover, there is evidence to suggest that the beneficial effects of thermal water continue for months following the completion of treatment. The mechanisms through which
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