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Key Documents

P5208

Sigma-Aldrich

Platelet-Derived Endothelial Cell Growth Factor human

≥97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Sinonimo/i:

PD-ECGF

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About This Item

Numero MDL:
Codice UNSPSC:
12352202
NACRES:
NA.77

product name

Platelet-Derived Endothelial Cell Growth Factor human, recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Origine biologica

human

Livello qualitativo

Ricombinante

expressed in baculovirus infected Sf21 cells

Saggio

≥97% (SDS-PAGE)

Forma fisica

lyophilized powder

Potenza

5-30 ng/mL EC50

PM

49 kDa

Confezionamento

pkg of 10 μg

Condizioni di stoccaggio

avoid repeated freeze/thaw cycles

tecniche

cell culture | mammalian: suitable

Impurezze

<1 EU/mg

N° accesso UniProt

Temperatura di conservazione

−20°C

Informazioni sul gene

Descrizione generale

Platelet-Derived Endothelial Cell Growth Factor (PD-ECGF) is an endothelial cell mitogen which was originally purified from human platelets. In contrast with the endothelial mitogens of the fibroblast growth factor (FGF) family, PD-ECGF is an endothelial cell growth factor initially purified from human platelets, which does not bind to heparin and does not stimulate the proliferation of fibroblasts. This is in contrast with the effect of PDGF, which stimulates the growth of human foreskin fibroblasts, but is inactive on endothelial cells. PD-ECGF will stimulate endothelial cells in vitro and in vivo. PD-ECGF is chemotactic for bovine aortic endothelial cells. PD-ECGF does not induce smooth muscle cell migration. PD-ECGF is involved in angiogenesis and has potent angiogenic activity both in the developing vascular system of the chick chorioallantoic membrane and in vascularization of tumors in nude mice. PD-ECGF has a pI of 4.0-4.8 and a molecular weight of 49 kDa.

The biological activity of recombinant, human PD-ECGF is measured in a cell proliferation assay using human umbilical vein endothelial cells. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay

Applicazioni

PD-ECGF is involved in angiogenesis and has potent angiogenic activity both in the developing vascular system of the chick chorioallantoic membrane and in vascularization of tumors in nude mice.

Azioni biochim/fisiol

Platelet-derived endothelial cell growth factor is a 45 kDa endothelial cell mitogen of pI 4.0-4.8 originally purified from human platelets. PD-ECGF is chemotactic for bovine aortic endothelial cells, however it does not induce smooth muscle cell migration. PD-ECGF is involved in angiogenesis and has potent angiogenic activity both in the developing vascular system of the chick chorioalantoic membrane and vascularization of tumor cells in nude mice. PD-ECGF is highly expressed in some tumors.

Stato fisico

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Risultati analitici

The proliferative activity of human PD-ECGF is measured in culture by using human umbilical vein endothelial cells.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


Certificati d'analisi (COA)

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H Aikawa et al.
Anticancer research, 19(5B), 4305-4309 (2000-01-11)
In order to elucidate the roles of tumor angiogenesis in lung carcinogenesis, the expressions of several angiogenic factors in lung carcinoma tissues were examined. Tissue specimens from 112 cases of resected non-small cell lung cancer (NSCLC) were studied. The expressions
Jue Li et al.
Bioengineered, 13(3), 4844-4856 (2022-02-15)
The association between spinal cord injury (SCI) and bladder symptoms has been intensively described. Human umbilical cord mesenchymal stem cell (hUC-MSC) treatment is beneficial to the recovery of bladder function after SCI, but its mechanism is unclear. We established an
L Griffiths et al.
British journal of cancer, 76(6), 689-693 (1997-01-01)
Angiogenesis plays an important role in the growth and metastasis of solid tumours. Platelet-derived endothelial cell growth factor (PD-ECGF) is known to be chemotactic for endothelial cells in vitro and angiogenic in vivo. It is also known as gliostatin, a
C-S Bao et al.
European review for medical and pharmacological sciences, 22(19), 6436-6447 (2018-10-20)
This research aimed to investigate the therapeutic effects of transplanted human umbilical cord mesenchymal stem cells (hUCMSCs) on spinal cord injury in mice and to explore its molecular mechanism. Spinal cord injury model in C57BL/6J mice was established. On the
Sinsuk Han et al.
Autophagy, 17(12), 4182-4201 (2021-03-25)
Mitochondria are the main cellular energy powerhouses and supply most of the energy in the form of ATP to fuel essential neuronal functions through oxidative phosphorylation (OXPHOS). In Alzheimer disease (AD), metabolic and mitochondrial disruptions are an early feature preceding

Articoli

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.

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