E1257

Epidermal Growth Factor from murine submaxillary gland

lyophilized powder, BioReagent, suitable for cell culture

• Sinonimo/i: EGF, Epidermal cell growth factor
• Numero CAS: 62229-50-9
• Numero CE: 263-468-7
• Numero MDL: MFCD01634807
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Origine biologica: mouse submaxillary glands
• Livello qualitativo: 200
• Nome Commerciale: BioReagent
• Saggio: ≥98% (SDS-PAGE)
• Forma fisica: lyophilized powder
• Potenza: 0.02-2.0 ng/mL EC₅₀
• Confezionamento: pkg of 0.1 mg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• tecniche: cell culture | mammalian: suitable
• Impurezze: endotoxin, tested
• Colore: white
• N° accesso UniProt: P01132
• Temperatura di conservazione: 2-8°C
• Informazioni sul gene: mouse ... Egf(13645)

Descrizione

Descrizione generale

Epidermal growth factor (EGF) is the member of EGF-like family of growth factors, that binds to EGF receptor. It is majorly expressed in kidney, parotid gland and salivary gland.

Applicazioni

Epidermal Growth Factor from murine submaxillary gland has been used

• in studying its effects on the development of caustic esophageal stricture in rats
• for preparation of MCF-10A cell line
• for the culture of primary hair follicle stem cells from arbas cashmere goat (gHFSCs) and adhesion of hair follicles (HF)

Azioni biochim/fisiol

Epidermal growth factor (EGF) blocks gastric acid and pepsin secretion and increases gastrointestinal cell proliferation. EGF stimulates proliferation and migration in bone marrow stromal cells (BMSC), that gives rise to osteoblasts, chondrocytes and adipocytes. It functions as a strong mitogenic factor for various cultured cells. Mutations in this gene is linked to hypomagnesemia. EGF plays a major role in the maintenance of hair and skin development.

Stato fisico

Lyophilized from 0.2 μm filtered solution in ammonium acetate buffer.

Nota sulla preparazione

Purified by gel filtration and ion-exchange chromatography.

Risultati analitici

The biological activity is measured in a proliferation assay using BALB/MK cells.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)