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N6658

Sigma-Aldrich

Nutrient Mixture F-12 Ham

With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sinonimo/i:

Ham’s F-12

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About This Item

Numero MDL:
MFCD00217417
Codice UNSPSC:
12352207
NACRES:
NA.75

product name

Nutrient Mixture F-12 Ham, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Livello qualitativo

400

Sterilità

sterile-filtered

Forma fisica

liquid

tecniche

cell culture | mammalian: suitable

Impurezze

endotoxin, tested

Componenti

L-glutamine: yes
HEPES: no
phenol red: yes
sodium pyruvate: 0.11 g/L
NaHCO3: yes

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Categorie correlate

Descrizione generale

Ham′s Nutrient Mixtures were mainly created to support the clonal growth of numerous clones of Chinese hamster ovary (CHO) cells and clones of HeLa and mouse L-cells. It is also used to grow primary rat hepatocytes and rat prostate epithelial cells. Ham′s F-12 is used as the medium of choice for a clonal toxicity assay using CHO cells.

Applicazioni

Nutrient Mixture F-12 Ham has been used:
  • to culture Chinese hamster ovary cells overexpressing insulin receptor (CHO-IR)
  • to culture LAN-1 cells
  • to maintain SUM149, SUM159, SUM190, FC-IBC02, BCX010, and IBC3 cells
  • with Dulbecco’s modified Eagle’s medium (DMEM) media in a 1:1 ratio to culture oral cavity squamous cell carcinoma (OSCC) cell lines.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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In vitro monolayer conditions are not able to reproduce the complexity of solid tumors, still, there is scarce information about the 3D cell culture models of endocrine tumor types. Therefore, our aim was to develop in vitro 3D tumor models
Kazuo Takazawa et al.
The Kobe journal of medical sciences, 54(1), E14-E22 (2008-09-06)
Insulin induces translocation of the glucose transporter GLUT4 from intracellular storage compartment to the plasma membrane via complex mechanisms that require intact cytoskeletal networks. In cultured adipocytes, conventional kinesin motor proteins have been proposed to mediate GLUT4 movements on microtubules.
Yoshinobu Takahashi et al.
Oncology letters, 14(1), 264-270 (2017-07-12)
Whether the poor prognosis of primary central nervous system lymphoma (PCNSL) compared with systemic diffuse large B cell lymphoma (DLBCL) is attributable to the immune privilege of the intracerebral location or to intrinsic differences in the biological characteristics of two
Toshimichi Tanaka et al.
Annals of surgical oncology, 26(5), 1401-1411 (2019-02-02)
Treatment-resistance genes limiting anticancer therapy have not been well clarified in colorectal cancer (CRC). We explored gene expression profiles to identify biomarkers for predicting treatment resistance to an anticancer drug in CRC. Six CRC cell lines were treated with phenylbutyrate
Jianjia Fan et al.
Journal of lipid research, 59(5), 830-842 (2018-03-23)
apoE is the primary lipid carrier within the CNS and the strongest genetic risk factor for late onset Alzheimer's disease (AD). apoE is primarily lipidated via ABCA1, and both are under transcriptional regulation by the nuclear liver X receptor (LXR).
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