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M8787

Sigma-Aldrich

Cloruro di magnesio

PCR Reagent, 25 mM MgCI2 solution for PCR

Sinonimo/i:

PCR optimization

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About This Item

Formula condensata:
MgCl2
Numero CAS:
7786-30-3
Peso molecolare:
95.21
Numero MDL:
MFCD00011106
Codice UNSPSC:
12352302
ID PubChem:
57654422
NACRES:
NA.52

Grado

PCR Reagent

Livello qualitativo

200
300

Stato

liquid

Confezionamento

vial of 1.5 mL

Concentrazione

25 mM±1 mM

tecniche

PCR: suitable

Colore

colorless

applicazioni

agriculture
diagnostic assay manufacturing

Attività estranea

DNase, RNase, none detected

Temperatura di conservazione

2-8°C

Stringa SMILE

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2
TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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Descrizione generale

Magnesium chloride solution is suitable for the optimization of polymerase chain reactions (PCR). Magnesium chloride acts as a source of magnesium ions for polymerase chain reactions (PCR). It influences the primer-template annealing temperature, fidelity, specificity, and yield. Excess amounts of magnesium can cause an increase in nonspecific products, while lack of magnesium can lead to reduced yield.

Applicazioni

Magnesium chloride solution has been used as a component of the:

  • amplification mixture for polymerase chain reaction (PCR)
  • amplification mixture in an inter simple sequence repeats (ISSR)-PCR
  • reaction buffer in 16S-23S rRNA gene internal transcribed spacer (ITS) PCR amplification and restriction analysis (ITS-PCR-RFLP)
  • PCR master mix for quantitative reverse transcription-polymerase chain reaction (qRT PCR) to amplify reversely transcribed cDNA
  • reaction mix for RT-PCR to assess the gene expression

Compatibilità

Suitable for optimization of polymerase chain reactions.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
SLCK6176
SLCH7551
SLCC7461
SLBZ9143
SLBQ4119V

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