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M1302

Sigma-Aldrich

M-MLV Reverse Transcriptase

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

Sinonimo/i:

Moloney Murine Leukemia Virus Reverse Transcriptase

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About This Item

Numero CAS:
9068-38-6
Numero MDL:
MFCD00283754
Codice UNSPSC:
12352202
NACRES:
NA.55

Origine biologica

Porcine intestinal mucosa

Livello qualitativo

200

Ricombinante

expressed in E. coli

Forma fisica

liquid

impiego

sufficient for 200 reactions
 sufficient for 250 reactions

Caratteristiche

dNTPs included: no
hotstart: no

Concentrazione

200 units/μL

tecniche

RT-PCR: suitable

Colore

colorless

input

purified RNA

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Descrizione generale

Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Applicazioni

M-MLV Reverse Transcriptase has been used:
  • for the preparation of cDNA libraries or for first strand cDNA synthesis
  • for use in a 2-step RT-PCR assay
  • in quantitative realtime-polymerase chain reaction (RT-qPCR)
  • in reverse transcription

Caratteristiche e vantaggi

  • Thermostable reverse transcriptase active at 37 °C.
  • Can be used to generate first strand cDNA of up to 7 kb.

Confezionamento

Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.

Definizione di unità

One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.

Nota sulla preparazione

The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.

Note legali

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Inibitore

N° Catalogo
Descrizione
Determinazione del prezzo

N8878

Nalidixic acid, ≥98%

N4382

Nalidixic acid sodium salt, powder

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Status epilepticus evokes prolonged increase in the expression of CCL3 and CCL4 mRNA and protein in the rat brain
<BIG><BIG>Guzik KA, et al.</BIG></BIG>
Acta Neurobiologiae Experimentalis, 71, 193-207 (2011)
Status epilepticus evokes prolonged increase in the expression of CCL3 and CCL4 mRNA and protein in the rat brain
Guzik-Kornacka A, et al.
Acta Neurobiologiae Experimentalis, 71(2), 193-207 (2011)
D S Howland et al.
Brain research. Molecular brain research, 11(3-4), 345-353 (1991-10-11)
The phosphoprotein synapsin I is expressed exclusively in neuronal cells. We are interested in elucidating the promoter sequences involved in cell type-specific expression of the synapsin I gene. The PC12 cell line expresses the 3.4 kb and 4.5 kb synapsin
Functional analysis of LHCSR1, a protein catalyzing NPQ in mosses, by heterologous expression in Arabidopsis thaliana
Dikaios I, et al.
Photosynthesis Research, 1-16 (2019)
G F Gerard et al.
DNA (Mary Ann Liebert, Inc.), 5(4), 271-279 (1986-08-01)
We have cloned and expressed in Escherichia coli a section of the Moloney murine leukemia virus (Mo-MLV) pol gene which includes the entire coding region of mature reverse transcriptase (RT) plus 284 additional base pairs 3' to the coding region
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Introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.

Reverse Transcription

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.

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