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63069

Sigma-Aldrich

Cloruro di magnesio

BioUltra, for molecular biology, ~1 M in H2O

Sinonimo/i:

Magnesium Chloride hydrate, Magnesium chloride (MgCl2)hexahydrate, MgCl2

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About This Item

Formula condensata:
MgCl2
Numero CAS:
Peso molecolare:
95.21
Numero MDL:
Codice UNSPSC:
12352302
ID PubChem:
NACRES:
NA.26

Grado

for molecular biology

Livello qualitativo

Nome Commerciale

BioUltra

Stato

liquid

Concentrazione

~1 M in H2O

tecniche

HPLC: suitable
flow cytometry: suitable

Impurezze

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.0002% total Nitrogen (N)

pH

5.0-7.5 (25 °C, 1 M in H2O)

Solubilità

water: soluble

Densità

1.08 g/mL at 20 °C

Anioni in tracce

phosphate (PO43-): ≤10 mg/kg
sulfate (SO42-): ≤50 mg/kg

Cationi in tracce

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤10 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤50 mg/kg
Li: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤500 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

1 M in H2O

Assorbanza UV

λ: 260 nm Amax: 0.05
λ: 280 nm Amax: 0.03

Compatibilità

suitable for molecular biology

Stringa SMILE

Cl[Mg]Cl

applicazioni

cell analysis
filtration
vaccine development

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2
TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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Descrizione generale

Magnesium chloride is widely used in polymerase chain reactions (PCR) buffers to supply magnesium ions. The concentration of Mg2+ is the most critical parameter that impacts both the efficiency and specificity of PCR when total genomic DNA is used, synthesis of long products, or during multiplex reactions. Elevated concentrations of Mg2+ enhance the stability of primer-template duplexes and the efficiency of amplification.

Applicazioni

Magnesium chloride solution has been used as a component of nuclei isolation buffer for in situ fixations and isolation of nuclei from Kupffer cells (or other liver cells).
Magnesium chloride solution has been used in the reaction mix of reverse transcriptase enzyme, PFV RT. It has been used in the preparation of artificial cerebrospinal fluid (ACSF).

Altre note

For additional information on our range of Biochemicals, please complete this form.

Prodotto comparabile

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves


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