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M5755

Sigma-Aldrich

MOPS-EDTA-Sodium Acetate (MESA)

powder for RNA electrophoresis buffers

Sinonimo/i:

MESA Buffer

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About This Item

Codice UNSPSC:
12161700
NACRES:
NA.25

Grado

for molecular biology

Livello qualitativo

200

Nome Commerciale

BioReagent

Forma fisica

powder

tecniche

electrophoresis: suitable

pH

6.8-7.2(reconstituted per directions)

Solubilità

H2O: soluble, faintly yellow

Attività estranea

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

Temperatura di conservazione

room temp

Categorie correlate

Descrizione generale

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Applicazioni

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Componenti

1x MESA buffer contains:
  • 40 mM MOPS
  • 10 mM sodium acetate
  • 1 mM EDTA (pH 8.3)

Ricostituzione

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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MOPS anhydrous, free-flowing, Redi-Dri™, ≥99.5%

Sigma-Aldrich

RDD018

MOPS

MOPS, Free Acid, Molecular Biology Grade CAS 1132-61-2 is a zwitterionic buffer useful in the pH range of 6.5-7.9. Has a pKa of 7.20 at 25°C.

Millipore

475922

MOPS, Free Acid, Molecular Biology Grade

MOPS anhydrous, free-flowing, Redi-Dri™, ≥99.5%

Sigma-Aldrich

RDD003

MOPS

Kathryn M Jones et al.
Journal of bacteriology, 184(9), 2491-2499 (2002-04-12)
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Protocolli

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

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