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Key Documents

M4892

SAFC

McCoy′s 5A Medium

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Produzione farmaceutica

Sinonimo/i:

5A basal medium

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About This Item

Numero MDL:
Codice UNSPSC:
12352207
NACRES:
NA.71

Livello qualitativo

Forma fisica

powder

tecniche

cell culture | mammalian: suitable

Componenti

phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Descrizione generale

McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.

Applicazioni

McCoy′s 5A Medium has been used for culturing:
  • human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
  • human colorectal HCT116 cells to study miR-224 over-expression in cancer
  • osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media

Azioni biochim/fisiol

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Quantità

Formulated to contain 11.9 grams of powder per liter of medium.

Ricostituzione

Supplement with 2.2 g/L sodium bicarbonate.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3


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Oncotarget, 8(53), 91134-91149 (2017-12-07)
Human osteosarcoma is considered a malignant tumor with poor prognosis that readily metastasizes. Tetrahydrocurcumin (THC) has been reported to have anti-tumor activity in numerous tumors. In addition, hypoxia-inducible factor-1α (HIF-1α) has been demonstrated to be associated with tumor metastasis by
Yu Wang et al.
PloS one, 8(7), e68744-e68744 (2013-08-08)
MicroRNA-224 (miR-224) is frequently over-expressed in liver and colorectal cancers. We and others have previously described the role of miR-224 over-expression in cell proliferation in vitro but we have yet to identify the relevant miR-224 direct target. In this study
Ying Liu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 44(4), 1396-1410 (2017-12-05)
Osteosarcoma (OS) is the most common primary malignant tumor of bone tissue. Although treatment effectiveness has improved, the OS survival rate has fluctuated in recent years. Andrographolide (AG) has been reported to have antitumor activity against a variety of tumors.
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