M9309
McCoy′s 5A Medium
With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Sinonimo/i:
5A basal medium
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Nome del prodotto
McCoy′s 5A Medium, Modified, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Livello qualitativo
Sterilità
sterile-filtered
Stato
liquid
tecniche
cell culture | mammalian: suitable
Impurezze
endotoxin, tested
Componenti
NaHCO3: 2.2 g/L
L-glutamine: 0.21 g/L
phenol red: 0.011 g/L
glucose: 3.0 g/L (Dextro)
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
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Descrizione generale
The amino acid requirements for in vitro cultivation of Novikoff hepatoma cells were reported by McCoy and colleagues. These experiments were carried out using Basal Medium 5A, which was later modified to create McCoy′s 5A medium.
Applicazioni
McCoy′s 5A Medium has been used:
- to culture HCT-116?cells
- to culture clear cell renal cell carcinoma (ccRCC) cells Caki-1
- to maintain and culture human bone osteosarcoma Saos-2 cell line for in vitro cell studies
Azioni biochim/fisiol
McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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