M0268
Terreno essenziale minimo di Eagle
With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Sinonimo/i:
MEM
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About This Item
Codice UNSPSC:
12352207
NACRES:
NA.75
Prodotti consigliati
Livello qualitativo
Stato
powder
tecniche
cell culture | mammalian: suitable
Componenti
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
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Descrizione generale
Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.
Applicazioni
Minimum Essential Medium Eagle has been used:
- in the isolation and culture of primary hepatocytes
- to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand
- to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells
Caratteristiche e vantaggi
- Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells.
- The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture.
Quantità
Formulated to contain 9.5 grams of powder per liter of medium.
Ricostituzione
Supplement with 2.2 g/L sodium bicarbonate.
Altre note
Essentially the same as the original formula, except it is provided as a powder.
Comunemente ordinati con questo prodotto
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
13 - Non Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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