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M1567

Sigma-Aldrich

Malic Dehydrogenase from porcine heart

≥600 units/mg protein (biuret), ammonium sulfate suspension

Sinonimo/i:

L-Malate: NAD+ oxidoreductase, MDH, Malate Dehydrogenase

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About This Item

Classificazione EC (Enzyme Commission):
1.1.1.37 (BRENDA, IUBMB)
Numero CE:
232-622-5
Numero MDL:
MFCD00131567
Codice UNSPSC:
12352204
NACRES:
NA.54

Stato

ammonium sulfate suspension

Livello qualitativo

200

Attività specifica

≥600 units/mg protein (biuret)

Attività estranea

Glutamic-Oxalacetic Transaminase ≤0.01%
Glutamic-Pyruvic Transaminase ≤0.01%

Temperatura di conservazione

2-8°C

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Descrizione generale

Malic Dehydrogenase is a ubiquitous enzyme, which exists in two isoforms in eukaryotic cells.
Malic dehydrogenase exists as a dimer with each subunit containing an NAD-binding domain and a substrate-binding carboxy-terminal domain required for activity.
Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Applicazioni

Malic Dehydrogenase from porcine heart has been used:
  • in qualitative protein binding measurements
  • to test internally calibrated electrochemical continuous enzyme assay (ICECEA) with model enzyme pair
  • to investigate the effect of chaperone on the refolding of heat-denatured malate dehydrogenase

Malic dehydrogenase has been used in a study to assess the effect of an immunomodulator S2 complex on the enzymes of the parasites. It has also been used in a study to investigate the heterogeneity of lactic and malic dehydrogenase in cerebrospinal fluid.

Azioni biochim/fisiol

Malic Dehydrogenase (MDH) plays an important role in the citric acid cycle in mitochondria. It catalyzes the interconversion of substrates malate and oxaloacetate with the simultaneous oxidation/reduction of NAD/NADH+. MDH present in the cytosol is involved in the shuttling of malate/aspartate.

Definizione di unità

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25°C.

Stato fisico

Suspension in 2.8 M (NH4)2SO4 solution, pH 6.0

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
SLCD5169
SLBT8312
SLBR6342V
SLBN8233V
SLBB2922V

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Yassir M Al-Mulla Hummadi et al.
Experimental parasitology, 112(2), 85-91 (2005-11-09)
S(2) complex has been reported to have a direct antileishmanial effect. The possibility that the direct antileishmanial effect may be due to inhibition of key enzymes involved in glucose metabolism and/ or enzymes associated with virulence was investigated. Cell pellets
Heterogeneity of lactic and malic dehydrogenase in cerebrospinal fluid
Lowenthal, A., et al.
Journal of Neurochemistry, 7, 135-140 (2006)
Electrochemical Coupled-Enzyme Assays at Carbon Nanotubes
Zhang M, et al.
Analytical Chemistry, 86(18), 9330-9334 (2014)
Malate dehydrogenases-structure and function
Minarik P, et al.
General Physiology and Biophysics, 21(3), 257-266 (2002)
Sensing NADH conformation using phasor analysis on fluorescence spectra
Palo D, et al.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 186(3), 105-111 (2017)
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Articoli

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocolli

Enzymatic Assay of Malic Dehydrogenase (EC 1.1.1.37)

Enzymatic Assay of Malic Dehydrogenase (EC 1.1.1.37)

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