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L2500

Sigma-Aldrich

L-Lactic Dehydrogenase from rabbit muscle

Type II, ammonium sulfate suspension, 800-1,200 units/mg protein

Sinonimo/i:

Lactate, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD

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About This Item

Numero CAS:
9001-60-9
Classificazione EC (Enzyme Commission):
1.1.1.27 (BRENDA, IUBMB)
Numero CE:
232-617-8
Numero MDL:
MFCD00131466
Codice UNSPSC:
12352204
NACRES:
NA.54

Tipo

Type II

Livello qualitativo

200

Forma fisica

ammonium sulfate suspension

Attività specifica

800-1,200 units/mg protein

PM

140 kDa

Attività estranea

pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

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Descrizione generale

Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate.
Isoelectric point: 8.4-8.6
Optimal pH : 7.5 .

Applicazioni

L-Lactic Dehydrogenase from rabbit muscle has been used:
  • for protein-binding measurement
  • to detect the action of oxaloacetate decarboxylase
  • to determine serum L- and D-lactate

Azioni biochim/fisiol

Lactic dehydrogenase is responsible for the conversion of pyruvate to lactate in the fermentative metabolism.
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Definizione di unità

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Stato fisico

Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0

Risultati analitici

Protein determined by biuret.

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Nutritional Biochemistry, 233-233 (1999)
Autofluorescence from NADH conformations associated with different metabolic pathways monitored using nanosecond-gated spectroscopy and spectral phasor analysis
Maltas J, et al.
Analytical Chemistry, 87(10), 5117-5124 (2015)
Anaerobic growth of Escherichia coli on D-tartrate depends on the fumarate carrier DcuB and fumarase, rather than the L-tartrate carrier TtdT and L-tartrate dehydratase
Kim OB, et al.
Archives of Microbiology, 188(6), 583-589 (2007)
José L Vasconcelos-Lima et al.
Genetics and molecular biology, 44(4), e20210127-e20210127 (2021-11-23)
Variations in dissolved oxygen levels are common in the Amazonian aquatic environments and the aquatic organisms that inhabit these environments developed a variety of adaptive responses to deal with such conditions. Some Amazonian fish species are tolerant to low oxygen
Duration of time that beef cattle are fed a high-grain diet affects the recovery from a bout of ruminal acidosis: Short-chain fatty acid and lactate absorption, saliva production, and blood metabolites
Schwaiger T, et al.
Journal of Animal Science, 91(12), 5743-5753 (2013)
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Articoli

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

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How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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