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L7651

Sigma-Aldrich

Lisozima

lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein, for molecular biology

Sinonimo/i:

Mucopeptide N-acetilmuramoil idrolasi, Muramidasi

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About This Item

Numero CAS:
12650-88-3
Classificazione EC (Enzyme Commission):
3.2.1.17 (BRENDA, IUBMB)
Numero CE:
235-747-3
Numero MDL:
MFCD00131557
Codice UNSPSC:
12352204

Grado

for molecular biology

Forma fisica

lyophilized powder

Attività specifica

≥40,000 units/mg protein

PM

single-chain 14.3 kDa

Composizione

protein, ≥90%

N° accesso UniProt

P00698

Temperatura di conservazione

−20°C

Informazioni sul gene

chicken ... LYZ(396218)

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Applicazioni

L'enzima rompe le pareti cellulari dei batteri; è utilizzato per preparare gli sferoplasti.
Lysozyme hydrolyzes the β-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine, a polysaccharide backbone of peptidoglycans in the cell wall structure of many microorganisms. This is particularly useful for lysing Gram-positive and Gram-negative bacteria for subsequent nucleic acid extraction.

Azioni biochim/fisiol

Il lisozima idrolizza i legami β(1→4) tra l′ acido N-acetilmurammico e i residui di N-acetil-D-glucosammina nel peptidoglicano e tra i residui di N-acetyl-D-glucosammina nella chitodestrina. Le cellule Gram-positive sono molto sensibili a questa idrolisi poiché le loro pareti cellulari hanno un'elevata percentuale di peptidoglicano. I batteri Gram-negativi sono meno sensibili in quanto posseggono una membrana esterna e hanno una percentuale inferiore di peptidoglicano. Tuttavia, queste cellule possono essere idrolizzate in presenza di EDTA che chela gli ioni metallici nella membrana esterna.

L'enzima è attivo in un ampio intervallo di pH (da 6,0 a 9,0). A pH 6,2, l'attività massima si osserva su una gamma più ampia di forze ioniche (da 0,02 a 0,100 M) rispetto a pH 9,2 (da 0,01 a 0,06 M).

Caratteristiche e vantaggi

  • Highly purified by repeated crystallization and dialysis
  • Each lot is use-tested for isolation of plasmid DNA from E. coli

Definizione di unità

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Stato fisico

Lyophilized powder, essentially salt-free

Nota sulla preparazione

3× crystallized

Sostituito da

N° Catalogo
Descrizione
Determinazione del prezzo

Substrato

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Lysozyme, Chicken Egg White Native, chicken egg white lysozyme. Inhibits the biological activity of lipopolysaccharides from periodontopathic bacteria.

Millipore

4403-M

Lysozyme, Chicken Egg White

Lysozyme from human neutrophils ≥95% (SDS-PAGE), lyophilized powder, ≥100,000 units/mg protein (E1%/280)

Sigma-Aldrich

L8402

Lysozyme from human neutrophils

SAFC

SAFC

1.05281

Lysozyme

Lisostafina lyophilized powder, Protein 50-70 % by biuret, ≥500 units/mg protein

Sigma-Aldrich

L7386

Lisostafina

Achromopeptidase from bacteria lyophilized powder, ≥1,000 units/mg solid

Sigma-Aldrich

A3547

Achromopeptidase from bacteria

Millipore

Millipore

71412

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Lisozima VETRANAL®, analytical standard

Supelco

34046

Lisozima

Kit DNA genomici batterici GenElute™ sufficient for 10 purifications

Sigma-Aldrich

NA2100

Kit DNA genomici batterici GenElute™

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How can I reconstitute Lysozyme from chicken egg white, Product L7651?

    For E. coli cell lysis, use a freshly prepared lysozyme solution (10 mg/mL) in 10 mM Tris-HCl, pH 8.0. The product is also soluble in water (10 mg/mL) yielding a clear to slightly hazy colorless solution.

  4. How stable are solutions of Lysozyme from chicken egg white, Product L7651?

    Aqueous solutions should retain activity for at least one month when stored between 2-8 °C.

  5. Is Lysozyme from chicken egg white, Product L7651, suitable for bacterial lysis prior to DNA purification?

    Each lot is verified to be suitable for use as a lysing agent in purifying plasmid DNA from E. coli. You can also use this product in combination with the GenElute™ Bacterial Genomic DNA Kit (Product No. NA2100) to purify genomic DNA from Gram-positive bacteria.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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