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A3547

Sigma-Aldrich

Achromopeptidase from bacteria

lyophilized powder, ≥1,000 units/mg solid

Sinonimo/i:

ACP, Lysyl endopeptidase

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About This Item

Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

bacterial (LYSOBACTER SP. BACTERIA M497-1)

Livello qualitativo

200

Forma fisica

lyophilized powder

Attività specifica

≥1,000 units/mg solid

PM

27 kDa

tecniche

cell based assay: suitable

Compatibilità

suitable for cell lysis

applicazioni

diagnostic assay manufacturing

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Applicazioni

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections.

Azioni biochim/fisiol

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.
Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme.
pH Optimum for activity: pH 8.5 - 9
Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD600=0.6 over 2 hours at 37 °C.

Definizione di unità

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

Stato fisico

crude powder

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P342 + P311

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificati d'analisi (COA)

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Protocolli

Enzymatic Assay of Achromopeptidase

Enzymatic Assay of Achromopeptidase

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