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L3397

Sigma-Aldrich

LB Broth (Luria low salt)

Powder microbial growth medium

Sinonimo/i:

Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)

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About This Item

Codice UNSPSC:
41106200
NACRES:
NA.85

Grado

for molecular biology

Livello qualitativo

200

Sterilità

non-sterile

Forma fisica

powder

Composizione

NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

tecniche

microbiological culture: suitable

pH

6.8-7.2(1.55% solution)

Compatibilità

suitable for molecular biology
nonselective for Escherichia coli
nonselective for coliforms

applicazioni

food and beverages
microbiology

Temperatura di conservazione

room temp

Descrizione generale

Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.

Applicazioni

LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.

Caratteristiche e vantaggi

Luria′s low salt LB powder provides:
  • Standard formulation
  • A budget-friendly alternative to liquid
  • Easy scale-up using larger package sizes

Nota sulla preparazione

1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Ricostituzione

Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Difco Manual, 272-272 (1998)
Giorgia Manzo et al.
ACS infectious diseases, 7(8), 2310-2323 (2021-07-31)
Reliable antimicrobial susceptibility testing is essential in informing both clinical antibiotic therapy decisions and the development of new antibiotics. Mammalian cell culture media have been proposed as an alternative to bacteriological media, potentially representing some critical aspects of the infection
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