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Key Documents

L2542

Sigma-Aldrich

LB Broth (Miller)

Liquid microbial growth medium

Sinonimo/i:

Luria Broth Miller, Luria-Bertani broth, Miller

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About This Item

Codice UNSPSC:
41106200
NACRES:
NA.85

Grado

for molecular biology

Livello qualitativo

Sterilità

sterile; 0.2 μm filtered

Forma fisica

liquid

tecniche

microbiological culture: suitable

pH

6.5-7.2

applicazioni

microbiology

Temperatura di conservazione

room temp

Compatibilità

nonselective for Escherichia coli
nonselective for coliforms

Descrizione generale

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

Applicazioni

LBBroth (Miller) is suitable for:

  • non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
  • selective cultivation when appropriate antibiotics are added.
  • the cultivation of E-coli cells for anti-bacterial assays.

Caratteristiche e vantaggi

Miller′s LB liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Componenti

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificati d'analisi (COA)

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Articoli

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

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