I3786

Protease Inhibitor Cocktail

Animal-Free, DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use with mammalian cell and tissue extract, DMSO solution

• Sinonimo/i: Protease inhibitor
• Codice UNSPSC: 12352200
• NACRES: NA.77

Proprietà

• product name: Protease Inhibitor Cocktail, Animal Component Free, for use with mammalian cell and tissue extract, DMSO solution
• Livello qualitativo: 200
• Forma fisica: DMSO solution
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Components of this cocktail are of non-animal origin and no animal products were used in the component production process. All vessels and instruments used for the cocktail production are dedicated for animal component free production and have never encountered an animal product. Aprotinin used in this cocktail is a recombinant bovine protein expressed in plants (Nicotiana).

This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts.

Specificità

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases.

Applicazioni

Animal component free (ACF). Optimized and tested for general use. Can also be used for purification of histidine-tagged proteins, as chelating agents have been omitted.

Componenti

AEBSF
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

Stato fisico

The components in this ACF protease inhibitor cocktail do not originate from animal sources and can thus be used in the production of animal-free proteins.

This ACF cocktail is supplied as a ready-to-use solution in DMSO. One ml of the cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g (wet weight) of bovine liver, or 10 mL of cell lysate obtained from CHO cells at a cell density of 10⁸ cells per mL.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H315,H319
• Consigli di prudenza: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Classi di pericolo: Eye Irrit. 2 - Skin Irrit. 2
• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): 188.6 °F - closed cup
• Punto d’infiammabilità (°C): 87 °C - closed cup