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H6254

Sigma-Aldrich

Hyaluronidase from sheep testes

Type V, lyophilized powder, ≥1,500 units/mg solid

Sinonimo/i:

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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About This Item

Numero CAS:
37326-33-3
Classificazione EC (Enzyme Commission):
3.2.1.35 (BRENDA, IUBMB)
Numero CE:
253-464-3
Numero MDL:
MFCD00131351
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

sheep testis

Livello qualitativo

200

Tipo

Type V

Stato

lyophilized powder

Attività specifica

≥1,500 units/mg solid

PM

55 kDa

applicazioni

diagnostic assay manufacturing

Temperatura di conservazione

−20°C

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Descrizione generale

Hyaluronidase is present in high amount in testis as it aids fertilization of the mammalian egg. Mammalian hyaluronidase has a catalytic epidermal growth factor (EGF)-like domain and a C-terminal cysteine rich region.

Applicazioni

Hyaluronidase has been used:
  • as a component of enzyme mix for the isolation of sertoli and germ cells from seminiferous tubules
  • in the pre-treatment of deparaffinized adenomas sections for immunohistochemistry
  • in the digestion of umbilical cord blood vessel for the generation of umbilical cord stromal stem cell lines

Azioni biochim/fisiol

Hyaluronidase from sheep testis is commercially used to depolymerise hyaluronic acid and chondroitin-sulphate in connective tissue and cartilage respectively.
These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Definizione di unità

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Altre note

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
SLCK4168
SLCL4821
SLCG7935
SLBZ9983
SLBZ2126

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Collagenasi

Human umbilical cord stromal stem cell express CD10 and exert contractile properties
Farias VA, et al.
Placenta, 32(1), 86-95 (2011)
Structure of human hyaluronidase-1, a hyaluronan hydrolyzing enzyme involved in tumor growth and angiogenesis
Chao KL, et al.
Biochemistry, 46(23), 6911-6920 (2007)
Yan Liang et al.
Scientific reports, 7(1), 12148-12148 (2017-09-25)
Meniscus fibrochondrocytes (MFCs) may be the optimal cell source to repair non-healing meniscus injuries using tissue engineering strategies. In this study, we investigated the effects of mitotic divisions and oxygen tension on the plasticity of adult human MFCs. Our assessment
Cartilage-specific matrix protein chondromodulin-I is associated with chondroid formation in salivary pleomorphic adenomas: immunohistochemical analysis
Kusafuka K, et al.
The American Journal of Pathology, 158(4), 1465-1472 (2001)
Isolation of Sertoli cells from adult rat testes: an approach to ex vivo studies of Sertoli cell function
Anway MD, et al.
Biology of Reproduction, 68(3), 996-1002 (2003)
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