F4042
Monoclonal ANTI-FLAG® M5 antibody produced in mouse
clone M5, purified immunoglobulin, buffered aqueous solution
Sinonimo/i:
Anti-ddddk, Anti-dykddddk
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About This Item
Prodotti consigliati
Origine biologica
mouse
Coniugato
unconjugated
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
M5, monoclonal
Stato
buffered aqueous solution
Purificato mediante
using Protein A
Reattività contro le specie
all
Concentrazione
2-5 mg/mL
tecniche
western blot (chemiluminescent): 10 μg/mL
Isotipo
IgG1
Sequenza immunogenica
DYKDDDDK
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
Descrizione generale
Monoclonal ANTI-FLAG® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.
Method of purification - Protein A
Method of purification - Protein A
Immunogeno
FLAG; peptide sequence DYKDDDDK
Applicazioni
Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.
Monoclonal ANTI-FLAG® M5 antibody produced in mouse has been used in western blotting and immunofluorescence.
Stato fisico
Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)
Nota sulla preparazione
Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.
Altre note
Antibody is not calcium dependent.
Note legali
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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