F3040
Monoclonal ANTI-FLAG® M1 antibody
clone M1, purified immunoglobulin, buffered aqueous solution
Sinonimo/i:
Anti-ddddk, Anti-dykddddk
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About This Item
Prodotti consigliati
Origine biologica
(Bioreactor)
Coniugato
unconjugated
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
M1, monoclonal
Stato
buffered aqueous solution
Purificato mediante
using Protein A
Reattività contro le specie
all
Concentrazione
2-5 mg/mL
tecniche
western blot: 10 μg/mL
Sequenza immunogenica
DYKDDDDK
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
Descrizione generale
The ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.
Method of purification - Protein A
Method of purification - Protein A
Immunogeno
FLAG; peptide sequence DYKDDDDK
Applicazioni
Monoclonal ANTI-FLAG® M1 antibody is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunofluorescence (1 paper)
Learn more product details in our FLAG® applications portal.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunofluorescence (1 paper)
Learn more product details in our FLAG® applications portal.
Azioni biochim/fisiol
Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca2+-dependent; the complex dissociates in the absence of calcium ions.
Stato fisico
Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).
Nota sulla preparazione
Dilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.
Note legali
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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