CELLMM2
FLAG® M Purification Kit
For Mammalian expression systems.
Sinonimo/i:
Anti-ddddk, Anti-dykddddk
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About This Item
Codice UNSPSC:
12352200
NACRES:
NA.32
Prodotti consigliati
Livello qualitativo
tecniche
protein extraction: suitable
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Applicazioni
The FLAG Purification Kit utilizes CelLytic™ Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG® M2 affinity gel for affinity purification of active FLAG fusion proteins.
Learn more product details in our FLAG® application portal.
Learn more product details in our FLAG® application portal.
Caratteristiche e vantaggi
- Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins.
- ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations.
- Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).
Confezionamento
Sufficient for 3-5 uses of 1-ml affinity purification column.
Altre note
Kit contains CelLytic™ M for cell lysis and protein extraction from Mammalian cells.
Note legali
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
I componenti del kit sono disponibili anche separatamente
N° Catalogo
Descrizione
SDS
- C2978CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.SDS
- SAE0194Purified 3xFLAG® peptide, ≥95% (HPLC), lyophilized powderSDS
- A2220ANTI-FLAG® M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solutionSDS
- C2103Chromatography columns, general-purpose, volume 10 mL, Overall H 5 in.SDS
Codice della classe di stoccaggio
10 - Combustible liquids
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Contenuto correlato
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.
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